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Core Labs Changing Ketone Testing in July

The Core laboratories in the Department of Pathology and Laboratory Medicine will be switching ketone testing to Precision Xceed Pro Monitor instruments from Abbott Diabetes Care Ltd. beginning Tuesday, July 25.

Specifically, beta-hydroxybutyrate (BHB), the dominant ketone body produced in fasting states and diabetic ketoacidosis, will be quantitatively measured in whole blood. Measurement of BHB will replace qualitative testing for aceotoacetate via Acetest™ tablets (in serum and urine).

Manufacture of Acetest Reagent has been discontinued. The change offers in improvement in the detection of ketoacidosis due to the quantitative nature of the replacement test and the fact that BHB is the dominant ketone (BHB/acetoacetate ratio is approximately 3:1 to 7:1 in severe ketotic states).

Additionally, acetoacetate is relatively rapidly converted to beta-hydroxybutyrate, which means Acetest is subject to false negative results.

Only whole blood samples will be acceptable on the new BHB assay. The reference cutoff for BHB: <0.6 mol/L

If you have questions, please contact Kimia Sobhani, PhD, at kimia.sobhani@cshs.org.